CRISPR technology

CRISPR technology

US 11,319,533 A CRISPR nanocomplex for nonviral genome editing and a method for preparing the same. The nanocomplex has a size of several nanometers to several micrometers, enabling intracellular delivery without external physical stimulation, and can be utilized for genome editing through nonviral routes with respect to target genes in cells. Korea Advanced Institute of Science and Technology (Daejeon, S. Korea) Chung HJ, Kang YK 5/3/2022 US 11,316,812 CRISPR–Cas methods and compositions for targeting RNA molecules, which can be used to detect, edit or modify a target RNA. Salk Institute for Biological Studies (La Jolla, CA, USA) Hsu PD, Konermann S 4/26/2022 US 11,306,309 Methods for inducing CRISPR–Cas-based gene regulation of a target nucleic acid in a cell. The methods include using modified single-guide RNAs that enhance gene regulation of the target nucleic acid in a primary cell for use in therapy ex vivo or in a cell in a subject for use in therapy in vivo. The Board of Trustees of Stanford University (Stanford, CA, USA) Porteus MH, Hendel A, Clark J, Bak RO, Ryan DE, Dellinger DJ, Kaiser R, Myerson J 4/19/2022 US 11,306,305 CRISPR–Cas9 ribonucleoprotein compositions comprising chemically modified CRISPR RNA guide and trans-acting CRISPR RNA components. Also, methods of using the same. Board of Trustees of Southern Illinois University (Carbondale, IL, USA), The Royal Institution for the Advancement of Learning/McGill University (Montreal, Canada) Gagnon K, Damha M, Malek-Adamian E, Habibian M, O’Reilly DT, Kartje Z 4/19/2022 US 11,298,411 A method of treating a subject having a retroviral infection by administering a therapeutically effective amount of a composition comprising a vector encoding a CRISPR-associated endonuclease and at least two guide RNAs, wherein the guide RNAs are complementary to two target sequences spanning from the 5′ to 3′ LTRs of the sequence in the retrovirus, thereby eradicating the retroviral infection, and a method of immunizing a subject at risk of retroviral infection by administering a prophylactically effective amount of a composition comprising a CRISPR-associated endonuclease and two or more different guide RNAs. Temple University of the Commonwealth System of Higher Education (Philadelphia, PA, USA) Khalili K, Hu W 4/12/2022 US 11,293,023 A means to modulate gene expression in vivo in a manner that avoids problems associated with CRISPR endogenous protein knockout or knock-in strategies and strategies that provide for correction or alteration of single nucleotides. Dana-Farber Cancer Institute (Boston, MA, USA) Buckley D, Winter G, Phillips AJ, Heffernan T, Bradner J, Roberts J, Nabet B 4/5/2022 US 11,286,478 Systems, methods and compositions for targeting nucleic acids, in particular non-naturally occurring or engineered DNA-targeting systems comprising a novel DNA-targeting CRISPR effector protein and at least one targeting nucleic acid component such as a guide RNA. The Broad Institute (Cambridge, MA, USA), Massachusetts Institute of Technology (Cambridge, MA, USA) Zhang F, Zetsche B, Yan W, Sanjana NE, Jones S 3/29/2022

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